This question has experienced several rounds of closing and reopening:
The close justification is that the film is off-topic, with the reasons cited typically being that films with slightly advanced technology and espionage ("spy-fi") or cartoon talking animals are not on-topic.
While I would be in favor of expanding the scope to include some of these works, that is not the issue here.
The problem is that while the two elements mentioned above may not be sufficient for a work to be on-topic, their absence should not be necessary.
Penguins of Madagascar has plenty of sci-fi/fantasy elements above and beyond its spy-fi or cartoon animal nature.
There is a ray that turns people into monsters:
Skipper: You point that death ray away from Private right now!
Private: It's not a death ray, Skipper! He's gonna turn us into monsters!
The ray also causes penguins to sprout supernumerary appendages.
Dave: Yepper-doo! And what comes next, Dave? Invasion! Ahhh! Horrible mutant penguins released on the streets of New York City!
Skipper: Private, are you okay?
Skipper, Rico and Kowalski gasp in surprise and notice that Private has sprouted a hand out of his buttocks.
Kowalski: Whoa! Butt-hand! There’s a hand attached to his butt. That was not... that was not there before.
The polarity of the uglification ray can be reversed through cuteness, which frankly is more fantasy than sci-fi.
In order to reverse the ray, we would need to replace the Medusa Serum with a power source of almost immeasurable cuteness.
Personally, this last one convinces me that it is on-topic. It's very MLP, isn't it?
The Wiki insists that Octavius Brine was once human and turned into an octopus, but this is not confirmed elsewhere (I'd like to ask this question if the series is deemed on-topic, though).
It seemed to me that such a contentious issue called for a Meta consensus, so: Is Penguins of Madagascar on-topic?